Bent Honoré Lab

Research

The main focus of the laboratory is the identification, characterisation and functional analyses of differentially expressed proteins in a set of diseases. Such proteins may be biomarkers for the disease or suitable targets for drug treatment. The research is conducted along two lines using studies on pathological patient tissue as well as basic laboratory research. The tissue samples are collected and analysed in collaboration with a number of clinical departments covering especially cardiovascular diseases, eye diseases and cancer. In addition we perform basic scientific studies involving laboratory animals and cell culture models as well as molecular studies on selected proteins.

Research interests

  • Differential proteomics of diseases:
  • Cancers: Tissue analyses of Hodgkin lymphoma and non-Hodgkin lymphoma. Analyses on colon cancer tissue and model studies using cultivated cells
  • Eye diseases: Uveal melanoma, retinal detachment, diabetic retinal changes
  • Cardiovascular diseases: Abdominal aortic aneurysms, insufficiency of the heart
  • Functional studies of proteins: Cellular localization, tissue localization, identification of protein-binding partners, effects on cells.

Methodologies

  • Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)
  • Nano-Liquid chromatography - tandem mass spectrometry (NanoLC-MS/MS)
  • Cell-biology techniques, including cell culturing, immunofluorescence light microscopy, transfection and Western blotting
  • Recombinant DNA technology, including heterologous expression and purification of recombinant proteins from Esherichia coli
  • Biochemistry techniques, including column affinity-chromatographic purification of proteins

Collaborators and centres

  • Steven K. Fisher1,2 & Geoffrey P. Lewis1, 1Neuroscience Research Institute and 2Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, USA
  • Bertil Damato1 & Sarah E. Coupland2, 1St. Paul’s Eye Clinic, Royal Liverpool University Hospital and 2Department of Pathology, University of Liverpool, United Kingdom
  • Jan U. Prause1, Steffen Heegaard1,2 & Michael Larsen2, 1Eye Pathology Section, Department of Neuroscience and Pharmacology, University of Copenhagen, Denmark, 2Department of Ophthalmology, Glostrup Hospital, University of Copenhagen
  • Gregory Rice, UQ Centre for Clinical Research, University of Queensland, Australia
  • Francesco d’Amore & Peter Kamper, Department of Hematology, Aarhus Hospital, Aarhus University Hospital
  • Henrik Vorum, Department of Ophthalmology, Aalborg Hospital, Aarhus University Hospital
  • Jes S. Lindholt, Vascular Research Unit, Viborg Hospital
  • Sigitas Urbonavicius, Department of Vascular Surgery, Viborg Hospital
  • Ole Thorlacius-Ussing, Department of Surgery, Aalborg Hospital, Aarhus University Hospital
  • Jan Frystyk, Medical Research Laboratories and Department of Endocrinology and Internal Medicine, Aarhus University Hospital
  • Nakul Mandal, Section of Ophthalmology, Department of Clinical Sciences, Lund University, Sweden

Research group members

 

 

Group leader

Bent Honoré

Professor
M
H bldg. 1182, 024
P +4587167814
P +4523382288

Publications

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