Vladimir V. Matchkov Lab

Research

The lab research activities are focused on mechanisms regulating resistance of small arteries and, thus, important for the control of blood pressure and tissue perfusion. The key interests of the lab include the following research areas: (i) the mechanisms of intercellular communication in the vascular wall; (ii) the role of ?2 isoform of the Na,K-ATPase for vascular function in small arteries; and (iii) the mechanisms responsible for cardiovascular abnormalities associated with major depression. We study membrane transport in smooth muscle cells and its significance for intracellular ion homeostasis involved in several vascular pathologies. Our studies involve a broad spectrum of in vitro and in vivo techniques for vascular function studies, as well as electrophysiological and molecular biological methods. The group participates in the training of undergraduate and graduate students.

Research interests

  • Mechanism of vasomotion: Experimental validation of a hypothetical model for the initiation of synchronized contractions of vascular wall, vasomotion.
  • A Ca2+-activated Cl- conductance in smooth muscle cells: Searching for the molecular identity of a Ca2+-activated Cl- channel and associated proteins as well as investigation of its functional significance in the vascular wall.
  • Regulation of intercellular communication: Analyses of putative gap junction blockers and development of new strategies to manipulate intercellular communication in the vascular wall.
  • Vascular significance of the ?2 isoform Na,K-ATPase: Investigation of ?2 isoform based membrane micro-domains and their significance for arterial contraction and endothelium-dependent relaxation.
  • Excitation-transcription coupling: Investigation of the role of voltage-gated Ca2+ channels in controlling smooth muscle cells phenotype and arterial structure.
  • Vascular abnormalities in depression: Investigation of mechanisms responsible for abnormal vascular function associated with development of major depression and it treatment.

Methodologies

  • Conventional and single-channel patch clamp
  • Isometric and isobaric myography of isolated small blood vessels (typically around 0.2 mm)
  • Live-cell confocal imaging in intact blood vessels to detect changes in intracellular calcium
  • Membrane potential measurements in intact isolated blood vessel using sharp glass electrodes
  • siRNA based down-regulation of specific genes in blood vessels in vivo
  • Quantitative determinations of mRNA and protein in small blood vessels
  • Immunohistochemistry
  • Stereology of small blood vessels
  • Telemetry and tail-cuff techniques for blood-pressure measurements

Collaborators and centres

  • Alexander Chibalin, Department of Molecular Medicine and Surgery, Integrative Physiology, Karolinska Institutet, Stockholm, Sweden
  • Daniel E. Otzen, Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology and Genetics, Aarhus University
  • Finn Skou Pedersen, Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology and Genetics, Aarhus University
  • Jens Christian Brings Jacobsen, Danish National Research Foundation Centre for Cardiac Arrhythmias, Department of Biomedical Sciences, University of Copenhagen
  • Niels Christian Nielsen and Troels Skrydstrup, Center for Insoluble Protein Structures (inSPIN), Interdisciplinary Nanoscience Center (iNANO), Department of Chemistry, Aarhus University
  • Olga Tarasova, Department of Human and Animal Physiology, Faculty of Biology, M.V.Lomonosov Moscow State University, Moscow, Russia
  • Ove Wiborg, Department of Clinical Medicine, Centre for Psychiatric Research, Aarhus University Hospital
  • Rikke Nørregaard, Department of Clinical Medicine, Aarhus University Hospital
  • Yasser Ahmed Mahmmoud, Ulf Simonsen, Department of Biomedicine

Research group members

Group leader

Vladimir Matchkov

Associate professor
M
H bldg. 1160, 214
P +4587167723
P +4521834982

Publications

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