Morten Nielsen Lab
Morten S. Nielsen
The to major themes in our laboratory are i) intracellular trafficking of Vps10p domain receptors in normal and polarized cells and ii) intracellular receptor trafficking in cells expressing alpha-synuclein. The Vps10p domain receptors sortilin and sorLA are both multifunctional type-I receptors that display anterograde and retrograde trafficking between several of the intracellular organelles. Sortilin and sorLA are furthermore asymmetric localized in polarized tissues. Our aim is to characterize and understand sortilin and sorLA trafficking in primary brain cells and in polarized membranes and how this trafficking regulates the function lipoprotein lipase in the brain. The goal of the second project is to study how alpha-synuclein affects intracellular trafficking of membrane receptors in the endo-lysosomale network. Alpha-synuclein is the major protein in alpha-synucleinopathies that causes different neurodegenerative diseases including Parkinson Disease.
Imaging: Confocal Imaging, Live Imaging, High Content Screening, TIRF, super resolution imaging
Primary cell cultures: Hippocampal neurons, Astrocytes, Glia cells
Cell Biology: Western blotting, immunoprecipitations, metabolic labeling, siRNA, recombinant protein expression, subcellular fractionation.
COLLABORATORS & Centers
- Gunilla Olivecrona, Medical Biosciences, Umeå Universitet, Sweden
- Oddumund Bakke, Department of Molecular Biosciences, Olso University, Norway
- Peder Madsen, Anders Nykjær, Claus Munck Petersen, Simon Glerup, Steen Vang Petersen and Camilla Gustafsen, Department of Biomedicine, Aarhus University, Denmark