Mariola Monika Golas

Group Leader

Mariola Monika Golas
Associate Professor
More information

RESEARCH
DNA encodes the genetic information of all living organisms, and although the genome is basically identical in almost all cells of an individual, any given cell expresses only a subset of genes dependent on its level and type of differentiation. During development sequential differentiation of cells takes place by programmed developmental processes that give rise to a number of different differentiation levels such as embryonic stem cells, progenitor cells and mature cells. In mature cells, a cell type-specific gene expression profile is maintained. Complex macromolecular assemblies composed of a number of components play critical roles in determining gene expression. Disturbances of their cellular functions can result in severe diseases such as neurodegeneration and cancer. We are interested in understanding the exact nature of the cellular mechanisms and in particular the structural basis for the function of relevant macromolecular assemblies in health and disease.

RESEARCH INTERESTS
Physiological gene expression: molecular mechanisms that contribute to gene expression during development and in differentiated cells

Neurodegenerative diseases: molecular causes of neurodegenerative disorders and identification of target molecules for drug development

Cancer development in children: molecular causes of pediatric cancer types and identification of target molecules for drug development

Development of methods: approaches to broaden the applicability of molecular electron cryomicroscopy and to correlate the results with complementary techniques such as mass spectrometry

METHODOLOGIES
Genetic approaches: subcloning, PCR, genetic tagging

Cell biological approaches: culturing of bacteria, yeast and mammalian cells, fluorescence microscopy

Biochemical and functional approaches: purification of endogenous and recombinant proteins and macromolecular assemblies, chromatographic techniques, ultracentrifugation, denaturing and native gel electrophoresis, blotting techniques, functional assays, mass spectrometry

Structural approaches: molecular electron cryomicroscopy, single-particle image processing, immuno-labeling, homology modeling and molecular fitting

COLLABORATORS & Centers

  • Centre for Stochastic Geometry and Advanced Bioimaging (CSGB), Aarhus University, Denmark
  • Danish Center for Scientific Computing at Aarhus University, Denmark
  • Bjoern Sander, Institute of Clinical Medicine, Aarhus University, Denmark
  • Jens R. Nyengaard, Institute of Clinical Medicine, Aarhus University, Denmark
  • Eva B. Vedel Jensen, Department of Mathematical Sciences, Aarhus University, Denmark
  • Mads Nielsen, Department of Computer Science, Copenhagen University, Denmark

 RESEARCH GROUP MEMBERS
Andriy Voronovsky, PhD; Lin Lin, PhD

 

                                                          

Henvendelse om denne sides indhold: 
Revideret 03.08.2016