Per Höllsberg

Group leader

Per Höllsberg
Professor
More information, www.hollsberglab.au.dk

RESEARCH
The key topic of our research is how viruses interfere with their host cell and thereby cause disease. Our projects fall within two main categories:

1. Multiple sclerosis - etiology and pathogenesis
Multiple sclerosis (MS) is a chronic neurologic disease with pathological changes in the brain involving demyelination. The pathogenesis of MS is unknown, but it is clear that environmental factors are of major importance for its development. These factors include in particular Epstein Barr virus (EBV), but may also involve human herpesvirus (HHV) 6A/B, low level of vitamin D3 and smoking. Ongoing projects within this theme include: Vitamin D and immunoregulatory cells in MS;
EBV and B cells in MS;
Immune response to EBV in MS; HHV-6A/B and immune cells (see also below).

2. Herpesviruses and reprogramming of host cells
Herpesviruses are able to establish lifelong latency in the host. Our laboratory is studying strategies used by human herpesvirus 6A/B to circumvent the antiviral host cell responses. Ongoing projects within this theme include: Inhibition of apoptosis by herpesvirus 6A/B;
Modulation of p53 activity during herpesvirus 6A/B infection.

RESEARCH INTERESTS

  • Multiple sclerosis
  • Human herpesvirus 6A/B
  • Epstein-Barr virus
  • Immunoregulatory cells and vitamin D
  • CD46

METHODOLOGIES

The following techniques are used in the laboratory

•    Separation of immune cells from blood

•    Cell culturing

•    Viral expansion and infection

•    Various cell stimulation assay

•    Cytotoxicity

•    RNA purification

•    Genomic DNA purification

•    cDNA synthesis

•    PCR and real-time qPCR

•    Cloning of genes

•    Cellular cloning

•    Transfection

•    Reporter gene assays - luciferase

•    Apoptosis assays

•    Proliferation assays

•    Cell-cycle assays

•    Immunoprecipitation - IP

•    Western blotting - WB

•    ELISA

•    Multiplex Bead Immunoassay

•    Confocal laser scanning microscopy

•    Flow cytometry

•    Fluorescence-activated cell sorting (FACS)

RESEARCH GROUP MEMBERS
Postdocs, PhD-students, Master students, Bachelor students, and a technician.

 

 

Henvendelse om denne sides indhold: 
Revideret 03.08.2016