Bent Honoré Lab

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis of experimentally induced detachment of the rabbit retina. Eighteen protein spots were found to be significantly and at least twofold differentially expressed between the sham (A) and detached retina (B). Fifteen protein spots were successfully identified by nano-liquid chromatography – tandem mass spectrometry (nanoLC-MS/MS). The results are published in: Mandal N, Lewis GP, Fisher SK, Heegaard S, Prause JU, la Cour M, Vorum H, Honoré B. Protein changes in the retina following experimental retinal detachment in rabbits. Mol. Vis. 17, 2634-2648, 2011.

Group Leader

Bent Honoré
Professor
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RESEARCH
The main focus of the laboratory is the identification, characterisation and functional analyses of differentially expressed proteins in a set of diseases. Such proteins may be biomarkers for the disease or suitable targets for drug treatment. The research is conducted along two lines using studies on pathological patient tissue as well as basic laboratory research. The tissue samples are collected and analysed in collaboration with a number of clinical departments covering especially cardiovascular diseases, eye diseases and cancer. In addition we perform basic scientific studies involving laboratory animals and cell culture models as well as molecular studies on selected proteins.

RESEARCH INTERESTS
Differential proteomics of diseases:

Cancers: Tissue analyses of Hodgkin lymphoma and non-Hodgkin lymphoma. Analyses on colon cancer tissue and model studies using cultivated cells

Eye diseases: Uveal melanoma, retinal detachment, diabetic retinal changes

Cardiovascular diseases: Abdominal aortic aneurysms, insufficiency of the heart

Functional studies of proteins: Cellular localization, tissue localization, identification of protein-binding partners, effects on cells.

METHODOLOGIES
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)

Nano-Liquid chromatography - tandem mass spectrometry (NanoLC-MS/MS)

Cell-biology techniques, including cell culturing, immunofluorescence light microscopy, transfection and Western blotting

Recombinant DNA technology, including heterologous expression and purification of recombinant proteins from Esherichia coli

Biochemistry techniques, including column affinity-chromatographic purification of proteins

COLLABORATORS & Centers

Steven K. Fisher1,2 & Geoffrey P. Lewis1, 1Neuroscience Research Institute and 2Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, USA

Bertil Damato1 & Sarah E. Coupland2, 1St. Paul’s Eye Clinic, Royal Liverpool University Hospital and 2Department of Pathology, University of Liverpool, United Kingdom

Jan U. Prause1, Steffen Heegaard1,2 & Michael Larsen2, 1Eye Pathology Section, Department of Neuroscience and Pharmacology, University of Copenhagen, Denmark, 2Department of Ophthalmology, Glostrup Hospital, University of Copenhagen

Gregory Rice, UQ Centre for Clinical Research, University of Queensland, Australia

Francesco d’Amore & Peter Kamper, Department of Hematology, Aarhus Hospital, Aarhus University Hospital

Henrik Vorum, Department of Ophthalmology, Aalborg Hospital, Aarhus University Hospital

Jes S. Lindholt, Vascular Research Unit, Viborg Hospital

Sigitas Urbonavicius, Department of Vascular Surgery, Viborg Hospital

Ole Thorlacius-Ussing, Department of Surgery, Aalborg Hospital, Aarhus University Hospital

Jan Frystyk, Medical Research Laboratories and Department of Endocrinology and Internal Medicine, Aarhus University Hospital

Nakul Mandal, Section of Ophthalmology, Department of Clinical Sciences, Lund University, Sweden

RESEARCH GROUP MEMBERS
Maja Ludvigsen, PhD-student; Inge Kjærgaard Nielsen, technician; Mona Britt Hansen, technician

 

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Revideret 03.08.2016