Mette Madsen

Group Leader

?Mette Madsen
Associate Professor
More information

RESEARCH
This research group was established in 2001 and has extensive experience in the field of endocytic receptors, primarily the components of the so called cubam complex; cubilin and amnionless. We primarily analyze our target receptors in biochemical assay and using cell biology model studies. We aim to provide novel information about the role of these proteins as well as their potential co-receptor, the multiligand megalin receptor, during the human reproduction process and a subsequent pregnancy, e.g. which role they play in the human placenta. Furthermore, we aim to characterize the expression and localization of these proteins and describe a potential function in hitherto not investigated human expression sites, e.g. mammary tissue. In connection to this, a potential role during differentiation and proliferation of cancer cells will be investigated.

RESEARCH INTERESTS
Characterization of expression, localization and function of cubilin, amnionless and megalin in human placenta. Investigated using both human placental tissue as well as relevant cell lines. Possible role as nutrient providers during pregnancy, potential signaling functions, general mediator of endocytosis.

Characterization of expression, localization and function of cubilin, amnionless and megalin in human mammary tissue. Investigated using both human mammary tissue as well as relevant cell lines, including cancer cells.

Characterization of the functional cubam complex in human ileum and in connection to this investigation of the role of megalin.

Investigation of the composition, assembly and processing of the cubam complex. Which regions of cubilin and amnionless form the cubam complex, how and where do the complex assemble, critical mediators of assembly, processing of assembled complex and essential elements for this.

METHODOLOGIES
Purification of proteins from solubilised tissue (human, rat, mouse) by ligand-affinty chromatography

Purification of proteins from recombinant expression (E. coli, yeast or mammalian cells) by ligand-affinty chromatography

mRNA analyses using mRNA extracted and purified from either tissue or cells (RT-PCR, quantitative Real Time PCR, sequencing, siRNA knock down assays)

Protein analyses (Including SDS-PAGE, western blotting, deglycosylation experiments, precipitation experiments, Surface Plasmon Resonance analyses, light scattering analyses)

Immunohistochemical analyses of sections of paraffin-embedded tissue or cryo sections of perfused tissue

Transfection of mammalian cells with cDNA constructs

COLLABORATORS & Centers

  • Søren K. Moestrup, Department of Biomedicine, Health, AU
  • Erik Ilsø Christensen, Department of Biomedicine, Health, AU
  • Henrik Hager, Department of Pathology, Arhus University Hospital, Aarhus, Denmark
  • Ebba Nexø, Department of Clinical Biochemistry, Arhus University Hospital, Aarhus, Denmark
  • Niels Uldbjerg, Skejby Sygehus, Aarhus University Hospital, Aarhus, Denmark
  • Linton Traub, Department of Cell Biology and Physiology, University of Pittsburg, USA
  • Robert Doyle, Department of Chemistry, Syracuse University, USA
  • Bent Honoré, Department of Biomedicine, Health, AU
  • Marina Romero-Ramos, Department of Biomedicine, Health, AU
  • Boe Sandahl Sørensen, Department of Clinical Biochemistry, Arhus University Hospital, Aarhus, Denmark
  • Gregers Rom Andersen, Department of Molecular Biology, Science and Technology, AU
  • Christian Brix Folsted Andersen, Department of Biomedicine, Health, AU
  • Vibeke Jensen, Department of Pathology, Arhus University Hospital, Aarhus, Denmark
  • Puk Sandager, Skejby Sygehus, Aarhus University Hospital, Aarhus, Denmark
  • Oddmund Bakke, Department of Molecular Biosciences, Oslo University, Norway

Research Group Members

Louise Lund Andersen, PhD student

Tine Kjærgaard, PhD student

Gitte Fynbo Biller, technician

Mette Singers Johansen, technician

Henvendelse om denne sides indhold: 
Revideret 03.08.2016