Thomas Corydon Lab

Research

The lab was started in 1998 and our key interest covers two research areas: (i) To understand and describe the pathophysiological aspects of genetic inherited diseases, and (ii) to develop gene therapy for the treatment of selected human diseases. Our studies involve design of therapeutic RNA molecules followed by efficacy analysis in cell model systems. Moreover, we perform functional as well as cellular localisation analysis of variant proteins using ectopic gene expression in eukaryotic cells. The group participates in the training of bachelor, MSc and PhD students.

Research interests

  • Severe vision loss: Development of gene therapy for age-related macular-degeneration (AMD)
  • Mitochondrial dysfunction: Identification of mitochondrial factors predisposing for the neurodevelopmental disease SCAD insufficiency using RNAi and mass spectrometry
  • Abnormal water homeostasis: Pathophysiological aspects of neurogenic and nephrogenic diabetes insipidus
  • Neuro-degenerative disorder: Investigation of Hsp60 variants associated with hereditary spastic paraplegia
  • Brain development: Analysis of the BRD1 protein associated with schizophrenia
  • SLCO1B1 variation and methotrexate disposition: Crystal structure of the hepatic transporter protein OATP1B1

Methodologies

  • Eukaryotic expression systems
  • Disease models
  • Gene-transfer: Transfection (cDNA), transduction (viral particles, e.g. AAV)
  • Protein analysis: Western blotting, immunostaining, confocal laser scanning microscopy (CLSM), activity measurements, functional studies, in vitro transcription/translation assay
  • RNA analysis: RNA interference, design of shRNA, siRNA and miRNA clusters, RNA purification, cDNA synthesis, qPCR analysis, promoter studies
  • DNA: Cloning, DNA purification

Collaborators and centres

  • Julie Gehl, Department of Oncology, Herlev Sygehus, Denmark
  • Niels Gregersen and Peter Bross, Research Unit for Molecular Medicine, Skejby Sygehus, Denmark
  • Anders Børglum, Jacob G. Mikkelsen, Jane H. Christensen and Thomas G. Jensen, Department of Biomedicine, University of Aarhus, Denmark
  • Toke Bek, Department of Ophtalmologi, Århus Sygehus, Denmark
  • Carsten Bjarkam, Institute of Anatomy, University of Aarhus, Denmark
  • Charlotte Knudsen, Institute of Molecular Biology and Genetics, University of Aarhus, Denmark
  • Søren Rittig, Research Laboratory of Nephrology and Hypertension, Skejby Sygehus, Denmark
  • Yvan Arsenijevic and Corinne Kostic, Unit of Gene Therapy and Stem Cell Biology, Jules-Gonin Eye Hospital, University of Lausanne, Lausanne, Switzerland

Research group members

Group leader

Thomas Juhl Corydon

Professor MSO
M
H bygn. 1242, 263
P +4587167775
P +4528992179

Publications

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