Standard commercially available software, also used for Flow Cytometry analysis can be used to analyze your CyTOF data.

FlowJo and FCS Express can be used for basic analysis such as bivariate plots, histogram overlays, heatmaps and dose response.  

FlowJo has also plugins, which offer user-friendly versions of clustering algorithms such as ViSNE/tSNE,UMAP, Tri-MAP, FlowSOM and others.

Free software designed for MatLab and R can be found online, but requires installation of R or MatLab.

Check the links below for details and other 3rd party software:

Selected 3rd party Data Analysis Software

• FlowJo (R-Plugins)        (https://www.flowjo.com/)

• FCSExpress                 (https://denovosoftware.com/)

• OmiQ                          (https://www.omiq.ai/)

• Cytobank                    (https://www.cytobank.org/)

• Cyclospore                  (https://www.cytosplore.org/)

• Vortex                        (https://web.stanford.edu/~samusik/vortex/)

• Accense                     (http://cellaccense.com)

• Catalyst                     (R based tool)

• Spectre                      (R based tool)

Fluidigm Helios Software:

Normalization, concatenation, debarcoding, and FCS to TXT is available in the Fluidigm Helios software.This can be done by the staff on the instrument computer or a standalone  Data analysis workstation. A freestanding version is available for download online (ask MCU-team)

Maxpar Pathsetter:

Can be used on MP DIP Assay -stained samples to automatically analyze and identify 37 immune cell types. One license is present at the MCU.