MCU (Mass Cytometry Unit @ Aarhus University)

What service do we offer?

SMC and IMC are “fee for service” based and include staff operated sample acquisition, preprocessing and (temporary) storage of SMC/IMC raw data, assistance in experimental design and panel setup, staining protocols, assistance in data-analysis (to some degree) and training/educational activities. The IMC service is provided in collaboration with the CellX cooperative.

For SMC, cellular targets in liquid suspension are labeled with heavy metal isotope-tagged reagents including antibodies, nucleic acid intercalators and analogs, as well as other biochemical ligands.

Each reagent is detected and quantified with cytometry by time-of-flight mass spectrometry in the CyTOF system.

For IMC, fixed tissue samples on slides are stained for cellular and/or structural targets using the same reagents as outlined above, and ablated from the slide pixel by pixel (Hyperion; 1µm² resolution) and analysed in the CyTOF system. IMC allows the multiplex analysis of up to 35 markers in the same tissue section simultaneously.

We serve users from:

• Aarhus University
• Other universities
• Businesses and industry