Services
Comprehensive proteomics support from sample to insight
We provide end-to-end proteomics services covering sample preparation, high-resolution LC–MS/MS measurement, and advanced quantitative data analysis. Our facility supports discovery proteomics, targeted questions, post-translational modification studies, and microdissected tissue workflows.
1. Sample preparation
We offer high-quality preparation of diverse biological materials, ensuring clean, reproducible peptide samples optimized for mass spectrometry.
Standard workflows
- FASP (Filter-Aided Sample Preparation)
- In-gel digestion
- LCM-derived sample processing (laser-capture microdissected samples)
- Tryptic digestion and peptide cleanup
Advanced enrichment and labeling
- Phosphopeptide enrichment for phosphoproteomics (other enrichment strategies for post-translational modifications available after discussion)
- TMT labeling for multiplexed quantitative studies (available on a per-project basis)
2. LC–MS/MS acquisition
We generate high-resolution proteomic data using state-of-the-art liquid chromatography–tandem mass spectrometry (Vanquish Neo UHPLC coupled with Orbitrap Ascend or EASY nLC-1200 coupled with Orbitrap Fusion).
Capabilities
- Standard 30-minute LC–MS/MS gradients
- Flexible acquisition methods tailored to project needs
- Suitable for:
- Discovery proteomics
- Quantitative studies
- PTM-focused investigations
- Small or challenging samples (e.g., LCM)
- Single-cell proteome analysis
3. Quantitative data analysis
Our computational pipelines provide robust, publication-grade insights.
What we deliver:
- Quantitative DIA analysis (Spectronaut-based workflows)
- Protein and peptide identification
- Differential expression & statistical testing
- Data visualization and figure-ready plots
- Clear summaries suitable for interpretation and publication
4. Final deliverables
Every project includes complete, transparent data handover.
- RAW LC–MS/MS files
- Processed data reports (if analysis is included)
- Methods summaries for manuscript use
- Figure legends and ready-to-use graphics
- Support in data interpretation and manuscript preparation (when appropriate)
Our approach
We maintain high scientific standards through:
- Rigorous sample handling
- Optimized and validated LC–MS/MS methods
- Reproducible quantitative pipelines
- Clear communication and shared interpretation of results
Frontier in proteomics: Single-Cell Proteomics (SCP)
A new frontier in proteomics is Single-Cell Proteomics (SCP).
- SCP aims to unravel the diversity of protein expression within individual cells.
- Access to SCP is possible through CellX - The Danish Single Cell Examination Platform.
- Please contact us for more details.
Contact
For collaborative enquiries and proposal support.
