SCP

Single-Cell Proteomics (SCP)

SCP setup:

Vanquish Neo UHPLC coupled with Orbitrap Ascend mass spectrometer.

SCP workflow:

From cell suspension or tissue sections to downstream single-cell LC-MS analysis. 

Technology key features:

  • LC-MS based proteomics at single-cell level, antibody independent.
  • Semi-automated, nl-ul level in-situ sample preparation.
  • Compatible with both cell suspension and fresh frozen/ FFPE samples.
  • Approx. 1000-2000 protein IDs from a single cell.
  • Current throughput 30 samples per day (SPD).
  • Provide complementary data to scRNAseq.

How to access SCP?

For more information and access of SCP through CellX platform, please contact the Proteomics Core Facility at the Department of Biomedicine, AU.