MINFLUX
MINimal flourescence photon FLUXes nanoscopy - MINFLUX
© 2024 abberior
Traditional fluorescence microscopy is limited by the diffraction limit of light, which restricts resolution to about 200-300 nanometers. MINFLUX break this limit and allows imaging at much higher resolutions (down to a few nanometers).
MINFLUX key features:
- Tracks labeled molecules at high frequencies up to 10 kHz, resolving molecular motion every 100 μs
- Achieves localization precisions of 1-3 nm (3D) in biological samples on large fields of view (10 x 15 μm)
- Compatible with both live and fixed fluorescently labeled specimens. Multi-color imaging is possible.
Read detailed MINFLUX principle HERE.
Fig. Comparison between confocal and MINFLUX resolution. Individual RNA probes hybridized along an RNA strand.
© 2024 DaMBIC.
The MINFLUX system contains two pulsed excitation laser sources @ 561 nm and @ 640 nm allowing for imaging of fluorophores in the excitation range ~550 - 680 nm.
MINFLUX is built onto an IX83 Olympus microscope with a multicolor CoolLED illumination source.