Chromium Single Cell (SC)

Chromium SC

Single cell RNA sequencing (scRNA-seq) is an advanced and effective technique for uncovering biological complexities driven by cellular heterogeneity. Chromium single cell gene expression platform from 10X Genomics, enables robust mRNA profiling at the single-cell level.

Typical 10X Genomics Chromium Single Cell Gene Expression Workflow:

  • Preparation of single cells or nuclei Suspension
  • Partitioning cells into GEMs (Gel Bead-In Emulsions)
  • Barcode incorporation
  • cDNA synthesis and library preparation
  • Next-generation sequencing (NGS)

10X Genomics Chromium Single Cell Solutions:

  • Single cell gene expression Flex
  • Single cell gene expæression (3')
  • Single cell immune profiling (5')
  • Single cell ATAC
  • Single cell multiome

To learn more of the various Chromium single cell solutions and  to help design your own scRNA-seq experiments, check the 10X Genomics product comparison page.

Fig. Chromium X - flexible instrument that runs all 10X Genomics single cell assays

The next generation GEM-X technology is released for better cost-effectiveness, higher-through put and better recovery/sensitivity. Read more about GEM-X here.

How to access Chromium SC?

For access of Chromium SC services under the CellX platform please reach out to SC core KU BRIC.

Other CellX platform options:

MOMA single-cell core