MSI
MSI - Mass Spectrometry Imaging
TimsTOF fleX MALDI-2 instrument Source: Bruker Daltonics
The timsTOF fleX MALDI-2 is a high-performance mass spectrometer that integrates Trapped Ion Mobility Spectrometry (TIMS) with Matrix-Assisted Laser Desorption/Ionization (MALDI) imaging. The MALDI-2 post-ionization dramatically enhances sensitivity for a comprehensive, untargeted spatial view of biomolecules directly from tissue.
Key Features:
- Technology: Uses MALDI with MALDI-2 post-ionization to greatly boost signal, particularly for lipids and metabolites.
- 4D Resolution: Delivers high spatial resolution (~5-10 µm) paired with ion mobility separation, adding a fourth dimension (x, y, m/z, ion mobility) for superior molecular specificity.
- Sample Compatibility: Supports both FFPE and fresh-frozen tissues with dedicated protocols. Please contact us for more detailed discussion on sample prep protocols.
- Untargeted Discovery: Enables hypothesis-free imaging of thousands of molecules without pre-defined panels.
- Multimodal: The "FLEX" design allows seamless switching between MALDI and Electro-spray ionization (ESI) ion sources on a single instrument.
Multiomic Applications:
- Spatial Metabolomics & Lipidomics: Direct imaging of endogenous small molecules and lipids.
- Spatial Proteomics: Peptide and protein imaging from FFPE tissues via on-tissue digestion.
- Spatial Glycomics: Mapping of N-linked glycans.
Data Output: Generates high-dimensional datasets for visualization and analysis in specialized software, (e.g. SCiLS Lab) or more customed data analysis pipelines.
For the further information and updates, please visit Bruker Daltonics.