MerFISH
Multiplexed Error-Robust Fluorescence in situ Hybridization - MerFISH
MerFISH setup at DaMBIC
MerFISH technology key features:
- Advanced imaging-based platform for spatially resolved transcriptomics analysis.
- Compatible with a wide range of cell cultures and tissue samples, including formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissues.
- High-plex gene expression analysis, capable of profiling hundreds to thousands of RNA species simultaneously.
- Customizable panels to target specific genes of interest, providing flexibility for diverse research needs.
- Flexible microscope platform allowing various applications and need (resolution down to ~150 nm).
Explore the spatial transcriptomics capabilities with MerFISH
MerFISH combines the principles of fluorescence in situ hybridization (FISH) with multiplexed error-robust barcoding strategies. Sequential hybridization of probe sets, followed by imaging and signal decoding, allows for the precise identification of RNA species in a multiplexed manner.
Fig. The MerFISH workflow. Source: Chen, Kok Hao, et al. 2015. "Spatially resolved, highly multiplexed RNA profiling in single cells." Science.
The versatility and adaptability of MERFISH makes it a valuable tool for uncovering the complex molecular landscape of cells and tissues. See, among others, the following publications:
- Mapping disease-related changes in gene expression: Cadinu, Paolo, et al. 2024. Cell.
- Characterize subcellular compartmentalization of RNAs: Xia, Chenglong, et al. 2019. PNAS.