Jensen & Aagaard Lab

Research

The laboratory aims at developing tools for non-viral and viral gene therapy and for delivery of functional genes or RNA inhibitors into cells or animals in preclinical settings. The group has extensive expertise with lentiviral- and AAV-based vector design, and our experimental portfolio includes gene delivery to skin, muscle, liver, eye and brain tissue for a number of murine models including phenylketonuria (PKU) and growth hormone deficiencies (GHD). Moreover, we explore viral delivery of designer nucleases (such as CRISPR/Cas9) for gene editing and genomic engineering of adult and stem cells and for gene-correcting therapies in vivo. We also use viral transduction for efficient reprogramming of human fibroblasts to induced pluripotent stem (iPS) cells. Finally, the group explores microRNA (miRNA) biology for regulation of gene expression by RNA interference and the use of miRNAs and inhibitors in therapeutic settings.

Research interests

  • Gene therapy of PKU and other metabolic disorders
  • Gene therapy of Growth Hormone Deficiencies by electrotransfer and microencapsulation
  • Gene therapy for improved healing of bone and tendons
  • Gene repair and genome editing in tissue culture and animal models
  • Generation of iPS cells from patients suffering from metabolic disorders (SCADD, MADD and FAO)
  • MicroRNA biology and developments of vectors for miRNA inhibition
  • Gene silencing by RNA interference and development of tissue-specific RNAi vectors

Methodologies 

  • Gene transfer in cell culture and animal models
  • Viral and non-viral gene transfer
  • Production and use of retro-, lenti- and AAV-based viral vectors
  • Gene targeting by TALE endonucleases and CRISPR/Cas9 systems
  • RNA interference by miRNA-mimics and conventional shRNA technology
  • Cloning, RNA analysis, qPCR analysis, immunofluorescence, ELISA and western blotting

Collaborators and centres

  • Paolo Bartolini and Cibele Peroni, IPEN, University of Sao Paulo, Brazil
  • Beat Thöny, University of Zurich, Switzerland
  • Moustapha Kassem, The Molecular Endocrinology & Stem Cell Research Unit, Odense University Hospital
  • Niels Gregersen and Peter Bross, Research Unit for Molecular Medicine, Aarhus University Hospital
  • Christian Kroun Damgaard, Department of Molecular Biology and Genetics, Aarhus University
  • Christian Würtz Heegaard, Department of Molecular Biology and Genetics, Aarhus University
  • Kjeld Søballe, Department of Orthopaedics, Aarhus University Hospital
  • Keld Poulsen, Statens Serum Institut, Copenhagen
  • Mipsalus ApS, Hørsholm and Thomas Vorup-Jensen, Department of Biomedicine, Aarhus University
  • Mark Denham, Department of Biomedicine, Aarhus University
  • Anders Nykjær, Department of Biomedicine, Aarhus University
  • Yonglun Luo, Department of Biomedicine, Aarhus University
  • Frederik Dagnæs-Hansen, Department of Biomedicine, Aarhus University
  • Thomas Corydon and Jacob Giehm Mikkelsen, Department of Biomedicine, AU as part of “Gene Therapy initiative Aarhus” (Supported by Lundbeck foundation)

Research group members

Group leader

Lars Aagaard

Lektor
M
H bygn. 1240, 233
P +4587167769
P +4529903699

Publications

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Revideret 20.09.2017