Publications
Eyer, K., Kuhn, P. & Dittrich, P. S. (2011). A microfluidic device for highly parallel isolation and chemical analysis of single cells. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1011-1013). Chemical and Biological Microsystems Society.
Kuhn, P., Eyer, K., Allner, S., Lombardi, D. & Dittrich, P. S. (2011). A microfluidic vesicle screening platform: Monitoring the lipid membrane permeability of tetracyclines. Analytical Chemistry, 83(23), 8877-8885. https://doi.org/10.1021/ac201410m
Kuhn, P., Eyer, K., Robinson, T., Schmidt, F. I., Mercer, J. & Dittrich, P. S. (2012). A facile protocol for the immobilisation of vesicles, virus particles, bacteria, and yeast cells. Integrative Biology (United Kingdom), 4(12), 1550-1555. https://doi.org/10.1039/c2ib20181j
Eyer, K., Kuhn, P., Hanke, C. & Dittrich, P. S. (2012). A microchamber array for single cell isolation and analysis of intracellular biomolecules. Lab on a Chip, 12(4), 765-772. https://doi.org/10.1039/c2lc20876h
Kurth, F., Eyer, K., Franco-Obregón, A. & Dittrich, P. S. (2012). A new mechanobiological era: Microfluidic pathways to apply and sense forces at the cellular level. Current Opinion in Chemical Biology, 16(3-4), 400-408. https://doi.org/10.1016/j.cbpa.2012.03.014
Küster, S. K., Fagerer, S. R., Verboket, P. E., Eyer, K., Jefimovs, K., Zenobi, R. & Dittrich, P. S. (2012). A novel interface coupling droplet microfluidics with maldi-mass spectrometry. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 251-253). Chemical and Biological Microsystems Society.
Eyer, K., Stratz, S., Kuhn, P., Kuester, S. K. & Dittrich, P. S. (2012). Single cell ELISA. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 983-985). Chemical and Biological Microsystems Society.
Kuhn, P., Eyer, K. & Dittrich, P. S. (2012). Single cell-liposome fusion for delivery of molecules into the cytosol. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 710-712). Chemical and Biological Microsystems Society.
Robinson, T., Kuhn, P., Eyer, K. & Dittrich, P. S. (2012). Single isolated vesicles in microfluidic traps to study membrane protein kinetics. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1519-1521). Chemical and Biological Microsystems Society.
Eyer, K., Kuhn, P., Stratz, S. & Dittrich, P. S. (2013). A microfluidic chip for the versatile chemical analysis of single cells. Journal of visualized experiments : JoVE, (80), e50618. https://doi.org/10.3791/50618
Purkayastha, N., Eyer, K., Robinson, T., Dittrich, P. S., Beck, A. K., Seebach, D., Kolesinska, B. & Cadalbert, R. (2013). Enantiomeric and diastereoisomeric (mixed) L/D-octaarginine derivatives - A simple way of modulating the properties of cell-penetrating peptides. Chemistry and Biodiversity, 10(7), 1165-1184. https://doi.org/10.1002/cbdv.201300180
Paulite, M., Blum, C., Schmid, T., Opilik, L., Eyer, K., Walker, G. C. & Zenobi, R. (2013). Full spectroscopic tip-enhanced raman imaging of single nanotapes formed from β-Amyloid(1-40) peptide fragments. ACS Nano, 7(2), 911-920. https://doi.org/10.1021/nn305677k
Eyer, K., Stratz, S., Kuhn, P., Küster, S. K. & Dittrich, P. S. (2013). Implementing enzyme-linked immunosorbent assays on a microfluidic chip to quantify intracellular molecules in single cells. Analytical Chemistry, 85(6), 3280-3287. https://doi.org/10.1021/ac303628j
Küster, S. K., Fagerer, S. R., Verboket, P. E., Eyer, K., Jefimovs, K., Zenobi, R. & Dittrich, P. S. (2013). Interfacing droplet microfluidics with matrix-assisted laser desorption/ionization mass spectrometry: Label-free content analysis of single droplets. Analytical Chemistry, 85(3), 1285-1289. https://doi.org/10.1021/ac3033189
Robinson, T., Kuhn, P., Eyer, K. & Dittrich, P. S. (2013). Microfluidic trapping of giant unilamellar vesicles to study transport through a membrane pore. Biomicrofluidics, 7(4), Article 044105. https://doi.org/10.1063/1.4816712
Eyer, K., Robinson, T., Kuhn, P. & Dittrich, P. S. (2013). Quantitative analysis of multiple antibody-ligand interactions in a microchip using fluorescence polarization anisotropy. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 500-502). Chemical and Biological Microsystems Society.
Stratz, S., Eyer, K., Kurth, F. & Dittrich, P. S. (2013). Towards quantitative analysis of single E. coli lysates. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1045-1047). Chemical and Biological Microsystems Society.
Eyer, K., Paech, F., Schuler, F., Kuhn, P., Kissner, R., Belli, S., Dittrich, P. S. & Krämer, S. D. (2014). A liposomal fluorescence assay to study permeation kinetics of drug-like weak bases across the lipid bilayer. Journal of Controlled Release, 173(1), 102-109. https://doi.org/10.1016/j.jconrel.2013.10.037
Eyer, K., Herger, M., Krämer, S. D. & Dittrich, P. S. (2014). Cell-free microfluidic determination of p-glycoprotein interactions with substrates and inhibitors. Pharmaceutical Research, 31(12), 3415-3425. https://doi.org/10.1007/s11095-014-1431-2
Robinson, T., Verboket, P. E., Eyer, K. & Dittrich, P. S. (2014). Controllable electrofusion of lipid vesicles: Initiation and analysis of reactions within biomimetic containers. Lab on a Chip, 14(15), 2852-2859. https://doi.org/10.1039/c4lc00460d
Stratz, S., Eyer, K., Kurth, F. & Dittrich, P. S. (2014). On-chip enzyme quantification of single Escherichia coli bacteria by immunoassay-based analysis. Analytical Chemistry, 86(24), 12375-12381. https://doi.org/10.1021/ac503766d
Pabst, M., Fagerer, S. R., Köhling, R., Eyer, K., Krismer, J., Jefimovs, K., Ibáñez, A. J. & Zenobi, R. (2014). Quantification of saquinavir from lysates of peripheral blood mononuclear cells using Microarrays and standard MALDI-TOF-MS. Journal of The American Society for Mass Spectrometry, 25(6), 1083-1086. https://doi.org/10.1007/s13361-014-0875-2
Steinhoff, R. F., Krismer, J., Eyer, K., Fagerer, S. R., Ibàñez, A., Pabst, M. & Zenobi, R. (2014). Rapid estimation of the energy charge from cell lysates using matrix-assisted laser desorption/ionization mass spectrometry: Role of in-source fragmentation. Analytical Biochemistry, 447(1), 107-113. https://doi.org/10.1016/j.ab.2013.11.013
Eyer, K., Root, K., Robinson, T. & Dittrich, P. S. (2015). A simple low-cost method to enhance luminescence and fluorescence signals in PDMS-based microfluidic devices. RSC Advances, 5(17), 12511-12516. https://doi.org/10.1039/c4ra12676a
Eyer, K., Root, K., Verboket, P. E. & Dittrich, P. S. (2015). Detection of Infertility-related Neutralizing Antibodies with a Cell-free Microfluidic Method. Scientific Reports, 5, Article 16551. https://doi.org/10.1038/srep16551
Kolesinska, B., Eyer, K., Robinson, T., Dittrich, P. S., Beck, A. K., Seebach, D. & Walde, P. (2015). Interaction of β3/β2-peptides, consisting of Val-Ala-Leu segments, with POPC giant unilamellar vesicles (GUVs) and white blood cancer cells (U937) - A new type of cell-penetrating peptides, and a surprising chain-length dependence of their vesicle- and cell-lysing activity. Chemistry and Biodiversity, 12(5), 697-732. https://doi.org/10.1002/cbdv.201500085
Pabst, M., Benešová, I., Fagerer, S. R., Jacobsen, M., Eyer, K., Schmidt, G., Steinhoff, R., Krismer, J., Wahl, F., Preisler, J. & Zenobi, R. (2016). Differential Isotope Labeling of Glycopeptides for Accurate Determination of Differences in Site-Specific Glycosylation. Journal of Proteome Research, 15(1), 326-331. https://doi.org/10.1021/acs.jproteome.5b00899
Eyer, K., Doineau, R. C. L., Castrillon, C. E., Mottet, G., England, P., Godina, A., Brient-Litzler, E., Nizak, C., Jensen, A., Griffiths, A. D., Bibette, J., Bruhns, P. & Baudry, J. (2017). Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring. Nature Biotechnology, 35(10), 977-982. https://doi.org/10.1038/nbt.3964
Kuhn, P., Eyer, K. & Dittrich, P. S. (2018). A microfluidic device for the delivery of enzymes into cells by liposome fusion. Engineering in Life Sciences, 18(2), 149-156. https://doi.org/10.1002/elsc.201600150
Weingart, O. G., Eyer, K., Lüchtenborg, C., Sachsenheimer, T., Brügger, B., Van Oostrum, M., Wollscheid, B., Dittrich, P. S. & Loessner, M. J. (2019). In vitro quantification of botulinum neurotoxin type A1 using immobilized nerve cell-mimicking nanoreactors in a microfluidic platform. Analyst, 144(19), 5755-5765. https://doi.org/10.1039/c9an00817a
ElKhoury, M., Chenon, G., Griffiths, A. D., Baudry, J. & Eyer, K. (2019). Metabolic characterization of individual IGG-secreting cells. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 (pp. 22-23). Chemical and Biological Microsystems Society.
Gérard, A., Woolfe, A., Mottet, G., Reichen, M., Castrillon, C., Menrath, V., Ellouze, S., Poitou, A., Doineau, R., Briseno-Roa, L., Canales-Herrerias, P., Mary, P., Rose, G., Ortega, C., Delincé, M., Essono, S., Jia, B., Iannascoli, B., Richard-Le Goff, O. ... Brenan, C. (2020). Author Correction: High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics (Nature Biotechnology, (2020), 38, 6, (715-721), 10.1038/s41587-020-0466-7). Nature Biotechnology, 38(6), 756. https://doi.org/10.1038/s41587-020-0563-7
Heo, M., Chenon, G., Castrillon, C., Bibette, J., Bruhns, P., Griffiths, A. D., Baudry, J. & Eyer, K. (2020). Deep phenotypic characterization of immunization-induced antibacterial IgG repertoires in mice using a single-antibody bioassay. Communications Biology, 3(1), Article 614. https://doi.org/10.1038/s42003-020-01296-3
Bounab, Y., Eyer, K., Dixneuf, S., Rybczynska, M., Chauvel, C., Mistretta, M., Tran, T., Aymerich, N., Chenon, G., Llitjos, J. F., Venet, F., Monneret, G., Gillespie, I. A., Cortez, P., Moucadel, V., Pachot, A., Troesch, A., Leissner, P., Textoris, J. ... Védrine, C. (2020). Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap. Nature Protocols, 15(9), 2920-2955. https://doi.org/10.1038/s41596-020-0354-0
Gérard, A., Woolfe, A., Mottet, G., Reichen, M., Castrillon, C., Menrath, V., Ellouze, S., Poitou, A., Doineau, R., Briseno-Roa, L., Canales-Herrerias, P., Mary, P., Rose, G., Ortega, C., Delincé, M., Essono, S., Jia, B., Iannascoli, B., Richard-Le Goff, O. ... Brenan, C. (2020). High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics. Nature Biotechnology, 38(6), 715-721. https://doi.org/10.1038/s41587-020-0466-7
Kurth, F., Weingart, O. G., Eyer, K., Loessner, M. J. & Dittrich, P. S. (2020). Microfluidic liposome biosensor for detecting botulinum neurotoxin physiologicalactivity. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 973-974). Chemical and Biological Microsystems Society.
Kräutler, N. J., Yermanos, A., Pedrioli, A., Welten, S. P. M., Lorgé, D., Greczmiel, U., Bartsch, I., Scheuermann, J., Kiefer, J. D., Eyer, K., Menzel, U., Greiff, V., Neri, D., Stadler, T., Reddy, S. T. & Oxenius, A. (2020). Quantitative and Qualitative Analysis of Humoral Immunity Reveals Continued and Personalized Evolution in Chronic Viral Infection. Cell Reports, 30(4), 997-1012.e6. https://doi.org/10.1016/j.celrep.2019.12.088
Molari, M., Eyer, K., Baudry, J., Cocco, S. & Monasson, R. (2020). Quantitative modeling of the effect of antigen dosage on b-cell affinity distributions in maturating germinal centers. eLife, 9, 1-49. Article e55678. https://doi.org/10.7554/eLife.55678
Rybczynska, M., Baudry, J. & Klaus, E. (2020). The impact of frost-damage on the quality and quantity of the secreted antigen-specific IgG repertoire. Vaccine, 38(33), 5337-5342. https://doi.org/10.1016/j.vaccine.2020.05.066
Eyer, K., Castrillon, C., Chenon, G., Bibette, J., Bruhns, P., Griffiths, A. D. & Baudry, J. (2020). The quantitative assessment of the secreted IgG repertoire after recall to evaluate the quality of immunizations. Journal of Immunology, 205(4), 1176-1184. https://doi.org/10.4049/jimmunol.2000112
Subedi, N., Van Eyndhoven, L. C., Hokke, A. M., Houben, L., Van Turnhout, M. C., Bouten, C. V. C., Eyer, K. & Tel, J. (2021). An automated real-time microfluidic platform to probe single NK cell heterogeneity and cytotoxicity on-chip. Scientific Reports, 11(1), Article 17084. https://doi.org/10.1038/s41598-021-96609-9
Bounab, Y., Eyer, K., Dixneuf, S., Rybczynska, M., Chauvel, C., Mistretta, M., Tran, T., Aymerich, N., Chenon, G., Llitjos, J. F., Venet, F., Monneret, G., Gillespie, I. A., Cortez, P., Moucadel, V., Pachot, A., Troesch, A., Leissner, P., Textoris, J. ... Védrine, C. (2021). Author Correction: Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap (Nature Protocols, (2020), 15, 9, (2920-2955), 10.1038/s41596-020-0354-0). Nature Protocols, 16(8), 4108. https://doi.org/10.1038/s41596-021-00492-7
Bucheli, O. T. M., Sigvaldadóttir, I. & Eyer, K. (2021). Biochemical Analysis of Secreted Molecules by Individual Cells. In Biochemical Analysis of Secreted Molecules by Individual Cells (pp. 495-517) https://doi.org/10.1007/978-981-10-8953-4_48
Bucheli, O. T. M., Sigvaldadóttir, I. & Eyer, K. (2021). Measuring single-cell protein secretion in immunology: Technologies, advances, and applications. European Journal of Immunology, 51(6), 1334-1347. https://doi.org/10.1002/eji.202048976
Canales-Herrerias, P., Crickx, E., Broketa, M., Sokal, A., Chenon, G., Azzaoui, I., Vandenberghe, A., Perima, A., Iannascoli, B., Goff, O. R. L., Castrillon, C., Mottet, G., Sterlin, D., Robbins, A., Michel, M., England, P., Millot, G. A., Eyer, K., Baudry, J. ... Bruhns, P. (2022). High-affinity autoreactive plasma cells disseminate through multiple organs in patients with immune thrombocytopenic purpura. Journal of Clinical Investigation, 132(12), Article e153580. https://doi.org/10.1172/JCI153580
Bucheli, O. T. M. & Eyer, K. (2023). Insights into the relationship between persistent antibody secretion and metabolic programming: A question for single-cell analysis. Immunology Letters, 260, 35-43. https://doi.org/10.1016/j.imlet.2023.06.006
Portmann, K., Linder, A., Oelgarth, N. & Eyer, K. (2023). Single-cell deep phenotyping of cytokine release unmasks stimulation-specific biological signatures and distinct secretion dynamics. Cell Reports Methods, 3(7), Article 100502. https://doi.org/10.1016/j.crmeth.2023.100502
Subedi, N., Verhagen, L. P., de Jonge, P., Van Eyndhoven, L. C., van Turnhout, M. C., Koomen, V., Baudry, J., Eyer, K., Dolstra, H. & Tel, J. (2023). Single-Cell Profiling Reveals Functional Heterogeneity and Serial Killing in Human Peripheral and Ex Vivo-Generated CD34+ Progenitor-Derived Natural Killer Cells. Advanced Biology, 7(4), Article 2200207. https://doi.org/10.1002/adbi.202200207
Aymerich, N., Bucheli, O. T. M., Portmann, K., Eyer, K. & Baudry, J. (2024). A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level. In V. Taly, S. Descroix & K. Perez-Toralla (Eds.), Microfluidics Diagnostics: Methods and Protocols (pp. 141-162). Springer. https://doi.org/10.1007/978-1-0716-3850-7_9
Schlotheuber, L. J., Lüchtefeld, I. & Eyer, K. (2024). Antibodies, repertoires and microdevices in antibody discovery and characterization. Lab on a Chip, 24(5), 1207-1225. https://doi.org/10.1039/d3lc00887h
