Laser Speckle Contrast Imaging (LSCI) is a real-time imaging technique with high spatial and temporal resolution, which allows for visualization of microvascular perfusion in anaesthetized or alert animals. Speckle patterns are obtained when the biological tissue creates natural variance of backscattering of the laser signal, thus mirroring identifiable structures that can be detected using a camera. Dynamic speckles can be tracked in relation to static speckles, to track blood flow in a vessel.

For investigations including:

• stroke (might require insertion of intracranial window)
• heart disease
• neurovascular flow
• arthrosis upon neurosurgery
• sunburns
• diabetic ulcers

The wire myograph system is used to test velocity and intensity of muscle contraction in vessels as small as 60 µm. Vessel circumference is kept constant and tension and/or relaxation in response to added compounds can be registered.

For investigation of tissue including:

• aorta, basilar, coronary, mesentery and other small arteries
• veins
• lymph vessels
• tracheal rings and bladder rings

Ultrasound can be applied to visualize major arteries for subsequent functional assessment.

For assesment of:

• flow velocity
• strain

The Vevo F2 LAZR-X system is located in the Skou building animal facility, building 1115. Therefore, please familiarize yourself with guidelines and regulations regarding animals as well as user quarantine regulations.