Mitochondrial respiration
The Oroboros Oxygraph-2k (O2k) evaluates mitochondrial function and efficiency, i.e. respiration capacity, in mitochondrial preparations and living cells.
Applications in brief
Upon sample loading, predefined or adjusted Substrate-Uncoupler-Inhibitor-Titration (SUIT) protocols are applied in which substrates, inhibitors, and uncoupler, are titrated in a sequence to stimulate or inhibit the enzymes and complexes of the electron transfer pathways. This facilitates evaluation of different coupling and pathway control states and provides detailed information on function of the electron transfer pathway components and the phosphorylation system.
Features
- mitochondrial function/dysfunction
- oxygen consumption together with either ROS production, mitochondrial membrane potential, Ca2+ or ATP production
Which samples may I use?
Fresh tissue is recommended, although frozen tissue may be used upon thorough protocol optimization
- tissue homogenates
- isolated mitochondria
- living and permeabilized cells (fresh or cryopreserved)
- permeabilized muscle fibers
Location
The O2k equipment is located on the 4th floor in the Skou building. Therefore, thoroughly evaluate your experiment steps (e.g. equipment preparation, sample preparation, centrifugation) prior to experimental onset.
Training and Manual
Quick-guide can be found on-site.
Specifications
| Full equipment name | Oroboros Oxygraph-2k, Oroboros Instruments GmbH, Innsbruck, Austria |
| Additional features | Oroboros FluoRespirometer module for measurement of oxygen consumption together with either ROS production, mitochondrial membrane potential, Ca2+ or ATP production. |
FAQ
Equipment
- How should I clean the equipment after use?
- Thorough cleaning is highly important for system functioality. After use, please clean the equipment by carefully following the on-site quick-guide.
- Please leave the work station neat and clean for the next user.
Data
Publications
- How shall I refer to the equipment?
- Please ensure to acknowledge the Phenotyping Core Facility. For guidance consult Publications & Acknowledgements.
