Lasers | Filters | Examples of appropriate fluorochromes |
405 nm 121 mW | Violet Side Scatter 1 405/10 | Particles in the size range 40-150 nm |
Violet Side Scatter 2 405/9 | Particles in the size range 80-1000 nm | |
447/60 | Brilliant Violet 421, eFluor 450, SBV440 | |
488 nm 50 mW | Blue Side Scatter 488/10 | |
531/46 | VioBright FITC, Alexa Fluor 488, GFP, CFSE, Venus, Qdot525 | |
561 nm 35 mW | Yellow Side Scatter 561/10 | |
670/30 | PE, DiI, mCherry, Qdot585, Qdot 605, SBB615 | |
637 nm 100 mW | Red Side Scatter 638/10 | |
670/30 | APC, Alexa Fluor 647, Alexa Fluor 660, DiIc1 (5), SBV 570, SBB675 | |
710/46 | APC-Alexa Fluor 700, Alexa Fluor 700, Cy5.5, Qdot 705, SBV710 | |
792/64 | APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, PE-Cy7, Qdot 800, SBV 790, SBY 800 |