ImageStream
Lasers | Channel | Detection range | Examples of appropriate fluorochromes |
Camera 1 | 1 | 420-480 nm | Bright field |
405 nm Camera 2 | 7 | 430-505 nm | DAPI, Live Dead Violet, eFluor450, BV421 |
8 | 505-570 nm | AF430, Pacific Orange, Qdot 525, eFlour506 | |
9 | 570-595 nm | Qdot 565, Qdot 585, BV570 | |
| 10 | 595-642 nm | Qdot 605, Qdot 625, eFluor605, BV605 | |
11 | 642-745 nm | Qdot 705, eFluor650, BV650 | |
12 | 745-800 nm | Qdot 800 | |
488 nm Camera 1 | 2 | 480-560 nm | FITC, AF488, GFP, Syto, MitoTracker green |
3 | 560-595 nm | DsRed, Cy3, RPE | |
| 4 | 595-642 nm | 7-AAD, PE-texas Red, PI |
5 | 642-745 nm | PerCP, 7-AAD, PE-Cy5, Draq 5, PerCP-eFlour710 | |
6 | 745-800 nm | PE-Cy7, Dark field (SSC) | |
561 nm Camera 1 | 3 | 560-595 nm | AF546, AF555, eFlour570 |
| 4 | 595-642 nm | RFP, mCherry, AF 568, Texas Red, eFlour615 | |
| 5 | 642-745 nm | PE- AF647, PE-Cy5.5, Nile Blue | |
| 6 | 745-800 nm | PE-Cy7, PE-AF750, Dark field (SSC) | |
642 nm Camera 2 | 11 | 642-745 nm | Nile Blue, APC, AF647, Cy5, eFlour660, eFlour710 |
| 12 | 745-800 nm | APC-Cy7,DyLight 750, Darkfield (SSC) | |
