Lasers and Filters in the NovoCyte

Lasers

Filters

Examples of appropriate fluorochromes

 

 

 

405 nm

50 mW

445/45

V450, Brilliant Violet 421, Pacific Blue, Hoechst Blue, Alexa Fluor® 405, DAPI,
SBV440, SB436, eF450, SNV428

 

530/30

SBV515, eFlour506

 

572/28

BV570

615/24

BV605, SNV605, Qdot625, SBV610

 

675/30

BV650, eVolve655, SBV670

 

780/60

BV785, SNV786

 

488 nm

20 mW

530/30

FITC, GFP, CFSE, YFP,
AF488, Vio515, BB515, NB530,
CYTO 9, eF520

 

572/28

PE

 

615/24

PI, PE-CF594, PE-eF610, ECD, NB610/70s

 

675/30 Per-CP, PE-Cy5, 7-AAD

 

780/60

PE-Cy7

640 nm

40 mW

675/30

DRAQ7, Alexa Flour 647,
APC, eFlour660, eFlour670

780/60

APC-H7, nIR Live/Dead, APC-eF780,
APC-Fire750, APC-Vio770