Lasers | Filters | Examples of appropriate fluorochromes |
|
|
|
405 nm 50 mW | 445/45 | V450, Brilliant Violet 421, Pacific Blue, Hoechst Blue, Alexa Fluor® 405, DAPI, SBV440, SB436, eF450, SNV428 |
| 530/30 | SBV515, eFlour506 |
| 572/28 | BV570 |
615/24 | BV605, SNV605, Qdot625, SBV610 | |
| 675/30 | BV650, eVolve655, SBV670 |
| 780/60 | BV785, SNV786 |
| ||
488 nm 20 mW | 530/30 | FITC, GFP, CFSE, YFP, AF488, Vio515, BB515, NB530, CYTO 9, eF520 |
| 572/28 | PE |
| 615/24 | PI, PE-CF594, PE-eF610, ECD, NB610/70s |
| 675/30 | Per-CP, PE-Cy5, 7-AAD |
| 780/60 | PE-Cy7 |
640 nm 40 mW | 675/30 | DRAQ7, Alexa Flour 647, APC, eFlour660, eFlour670 |
780/60 | APC-H7, nIR Live/Dead, APC-eF780, APC-Fire750, APC-Vio770 |