Lasers and Filters in the Penteon
Laser and detector type
Lasers | Filters | Examples of appropriate fluorochromes |
349 nm 20,2 mW | 445/45 | BUV395, Hoechst, DAPI |
| 525/45 | BUV496 | |
| 586/20 | BUV563 | |
| 615/20 | ||
| 667/30 | BUV661 | |
725/40 | SBUV740, BUV737 | |
| 780/60 757LP | QDOT800, BUV805 | |
405 nm 100 mW | 445/45 | V450, Brilliant Violet 421, Pacific Blue, Hoechst Blue, Alexa Fluor® 405, DAPI, |
| 525/45 | SBV515, eF506 | |
| 586/20 | BV570 | |
| 615/20 | BV605, SNV605, Qdot625, SBV610 | |
| 667/30 | BV650, Qdot655, SBV670 | |
| 685-705 | Qdot705 | |
| 725/40 705LP | BV711, SB702 | |
| 780/60 757LP | BV785, SB780, Qdot800, SNV786 | |
488 nm 100 mW | 525/45 | FITC, GFP, CFSE, YFP, |
| 586/20 | ||
| 615/20 | NB610/70s | |
| 667/30 | ||
| 685LP 695/40 705LP | PerCP-Cy5.5, BB700 | |
| 725/40 | PerCP-eFlour710 | |
561 nm 100 mW | 586/20 | PE, RFP, tdTomato, AsRed, Cy3, DyLight550 |
| 615/20 | PI, PE-CF594, PE-Vio615, mCherry, ECD | |
| 667/30 | PE-Cy5, 7-AAD | |
| 685LP 695/40 705LP | PE-Cy5.5 The filter configuration in the Penteon makes this filter functionally 695/20 because of the two longpass filters. | |
| 725/40 | PE-Alexa Flour 700 | |
| 757LP 780/60 | PE-Cy7, PE-Vio770 | |
637 nm 100 mW | 667/30 | APC, Alexa Flour 647, eF670, eF660, |
685LP | Alexa Flour 680, DRAQ7 The filter configuration in the Penteon makes this filter functionally 695/20 because of the two longpass filters. | |
| 725/40 | Alexa Flour 700, DRAQ7 | |
| 757LP 780/60 | APC-H7, APC-eFlour 780, APC-Fire750, APC-Vio770, nIR Live/Dead |
