Lasers and Filters in the 4 Laser FACS Aria III

Lasers	Longpass Filters	Bandpass Filters	Examples of appropriate fluorochromes
405 nm 50 mW	-	450/40	V450, Brilliant Violet 421, Pacific Blue, Hoechst Blue, Alexa Fluor® 405, DAPI, SBV440, SB436, eF450, SNV428
	502	510/50	SBV515
	570	605/30	Brilliant Violet 605, SBV610, SNV605

488 nm 20 mW	502	530/30	FITC, GFP, CFSE, YFP AF488, Vio515, BB515, NB530 CYTO 9, eF520
	655	695/40	BB700 PerCP-Cy^TM5.5, PerCP

561 nm 50 mW	-	582/15	PE, AsRed, tdTomato, tRFP, DyLight550,
	600	610/20	PE-CF-594, PI, ECD Living Colors® mCherry, PE-Vio615
	630	670/14	PE-Cy^TM5, 7-AAD
	735	780/60	PE-Cy^TM7, PE-Vio770

633 nm 20 mW	-	660/20	APC, Alexa Flour® 647, eFlour660, eFlour670, DyLight633
	690	730/45	DRAQ7, Alexa Fluor® 700, Alexa Fluor® 680
	735	780/60	APC-Fire750, APC-H7, nIR Live /Dead, APC-Vio770, APC-eFlour780

Revised 02.01.2024

FACS Core Facility