Recent breakthroughs in single cell sequencing, single cell multi-omics, and spatially resolved transcriptomics and proteomics have refined our understandings of how cells interact within healthy and diseased tissues. To strengthen single cell and spatially resolved multi-omics research in Denmark and to lead the development of next generation precision medicine, a national-wide roadmap infrastructure CellX is established. This one-day workshop brings together leading scientists and biotech companies to share their latest development in single cell and spatially resolved multi-omics technologies and applications.